1. pMSCV-Mouse RORgt-EF1α-GFP-T2A-Puro lentivector plasmid
Quantity: 10 µg 2.pMSCV-Mouse RORgt-EF1α-GFP-T2A-Puro piggyBac transposon plasmid
Quantity: 10 µg 3.MSCV-Mouse RORgt-EF1α-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant)
Quantity: 5 x 10^6 Cells/mL
Advantage: Choose a mouse RORγt overexpression system with your choice of lentivector- or PiggyBac-based transgenesis, or RORγt-overexpressing Jurkat cells