Novel mAb Development

  • Novel mAb Development
Hybridoma Generation for Therapeutic Antibody Discovery

Hybridoma technology and development is a critical step on the path towards therapeutic mAb discovery and antibody drug lead generation. Our partner GenScript's Hybridoma Generation for Therapeutic Antibody Discovery Service starts by selecting from a portfolio of immunization approaches to insure robust and appropriate immune responses. The combination of optimized immunization, fusion, and functional screening assays yields up to 100 high quality clonal hybridomas, assuring the success of your antibody drug discovery from the very beginning.  

Key Features of Premium Hybridoma Development Services

 

1. One stop solution: from antigen production to hybridoma development and characterization.
2. Full spectrum of immunization approaches: protein, peptide, whole cells and DNA immunization.
3. High throughput screening
4. Comprehensive functional assay: Validated functional assay platforms provide reliable in vitro screening.
5. Readily integrated downstream services: Ab sequencing, Ab humanization, rAb production and anti-idiotype antibodies for your Ab drug PD/PK studies.   

 

Premium Hybridoma Development Service Details (SC1693) (Service Flowchart) 

 

 


 

 Premium hybridoma development service 

Phase Description
Immunogen Preparation

 

 

 

Protein immunogen supplied by GenScript:(Strongly Recommended)
  • GenScript generates high quality antigen with our gene synthesis and protein expression service

 

 

 

Or qualified immunogen provided by customer:
  • Protein antigen must be >85% purity, 7-10mg, GenScript will verify the antigen purity and quantity before immunization
Immunization
  • Immunizations for 50 Balb/c mice
  • Test bleed
  • Maintain the immunized animals (six-months)
Cell Fusion, Screening
  • Cell fusion, (4x10e4 clones)
  • Primary screen (NativeSelect™ ELISA or HT March™)
  • Expand all positive parental clones into 24-well plates
  • Re-screen the samples (NativeSelect™ ELISA or HT March™)
  • Pick and freeze positive clones from initiated screening (50-100 positive clones)
Superdoma™ Assay and Isotyping
  • Superdoma™ assay to sort top affinity clones from different epitope-binding groups
  • Isotyping all the clones
  • All positive clones for functional assay ( 50-100 clones)
Small Scale Antibody Production
  • Small scale antibody production ( 50-100 clones, supernatants or 1-5mg purified antibody for each)
First Delivery
  • Supernatants or purified mAbs for In-Vitro functional assay and affinity measurement
Functional Assay(Optional)
  • Functional assay according to customers'request
Subcloning, Expansion and Cryopreservation
  • Subclone and screen (NativeSelect™ ELISA or HT March™)
  • Freeze down multiple vials for each clone
Final Delivery
  • 2 vials of cell lines for 2 subclone from each parental clone and 5 ml supernatants
  • Full report
Large Scale Antibody Production and Storage Service(Optional)
  • Produce antibody with roller bottle culture or ascites production
  • Purify the produced antibodies by protein A/G affinity column
  • Verify the produced antibodies
  • Cell bank service
  • Antibody sequencing
  

Note: 
1) This protocol is for you reference, and we can customize your project based on your specific requirement. 
2) We provide cell line storage service.

 

 



For Quotations & Order Requests

Write to Us : info@ennovationlifesciences.com, ennovationlifesciences@gmail.com

Call Us : +91-9711347133, +91-11-42760231