Site-directed mutagenesis facilitates life science research by creating specific DNA mutations (including point mutations, deletion mutations, and insertion mutations) to enable studies of gene regulatory elements, DNA-protein interactions, protein structure/function, enzyme active sites, and novel proteins.


Our Site-directed mutagenesis services provide a fast, cost-saving, way to get 100% accurate mutant DNA clones, without having to spend your time and money on mutagenesis kits to do PCR mutagenesis, cloning, and sequencing in your own lab. Powered by our industry-leading gene synthesis technology and high-performance GS polymerase, GenScript can make any gene template and any number of mutations.

Advantages of our Site-Directed Mutagenesis Services

1. Unparalleled Accuracy: Our high performance GS polymerase ensures accuracy of mutants, avoiding creation of unwanted mutations.
2. Unlimited Sites: Using our sophisticated gene synthesis and mutagenesis technology, we can introduce mutations at any site.
3. Mutations on Large DNA Constructs: Our technology is optimized to introduce point mutations, insertion mutations, and deletion mutations on constructs as large as 12 kb (including target gene and its vector).
4. One-stop solutions: We offer comprehensive upstream and downstream services, including template DNA sequencing, de novo gene synthesis, Express Cloning into free expression vectors, customized vector construction, and protein expression and purification.
5. Most Competitive Prices
 
How to Submit DNA templates for Mutagenesis

The fastest, and usually the most cost-effective, way to get the mutant DNA constructs you need is to order Express Mutagenesis bundled with gene synthesis using our online quotation form.

1. The complete sequence of the template including the target gene and its vector
2. Mutation specifications
3. Maps and antibiotic resistance of the template and destination vectors
4. Recommended to send 4 μg of template DNA as the starting material. If the starting material is bacterial culture, please send the culture at sufficient amount.
 
Delivery Specifications for Mutagenesis Services

All of our mutagenesis services offer the following delivery package:

1. 4 μg lyophilized destination vector containing the gene
2. Sequence chromatograms covering your gene (electronic)
3. Construct map for the plasmid (electronic)
4. Quality assurance certificate
5. Mutagenesis Service Online Quotes & Orders