Process Development

With team of experienced scientists and State-of-the-art technology, We provides customized services for optimizing growth and production media for target cell lines, defining feeding strategies, studying cell line stability, and increasing production. We also offer a thorough analysis of growth rate, subcultivation ratios, cell mass, and other parameters. Upon request, we can perform additional specific process optimization studies or employ our scaled-down models to predict cell performance.

We can optimize protein purification and process development. For down-stream process, protein purification is typically conducted through affinity column, but we also offer other chromatographic methods including gel filtration, ion exchange, and hydrophobic interactions. The purification tags are removed via a specific protease if desired, and the protein is further purified by gel filtration.

Stable cell line development
codon optimization technology significantly increased expression level in any system.
1. Reliable DHFR system, used for many commercial products.
2. Professional high-throughput screening platform.
3. Strict protein expression stability and growth stability evaluation.

Upstream process development
1. Cell culture condition improvement
2. Feeding strategies optimization
3. Scale-up to bioreactor
4. Product quality control and optimization

Downstream process development
1. Purification process development
2. Protein modification process development
3. Analytical assay development